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TALL-104
TALL-104
規格:
價格:
編號:B165825
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
TALL-104
商品貨號
B165825
Organism
Homo sapiens, human
Tissue
peripheral blood
培養基
Cell Type
T lymphoblast
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
acute lymphoblastic leukemia
Age
2 years
Gender
male
Storage Conditions
liquid nitrogen vapor phase
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Karyotype
46, XY; t(11;14)(p13;q11)
Images
Cell Micrograph of ATCC CRL-11186, TALL-104
Derivation
The TALL-104 line was established from the peripheral blood of a child in relapse with T-ALL.
Clinical Data
male
peripheral blood of a child in relapse with T-ALL
Antigen Expression
CD2 +; CD3 +; CD7 +; CD8 +; CD56 +; CD4 -; CD16 -
Receptor Expression
T cell antigen receptor alpha/beta (TCR), expressed
interleukin 2 (IL-2), expressed
interleukin 2 (IL-2); T cell antigen receptor alpha/beta (TCR)
Genes Expressed
interferon gamma (IFN gamma); tumor necrosis factor alpha (TNF alpha); granulocyte monocyte colony stimulating factor (GM-CSF)
Cellular Products
interferon gamma (IFN gamma); tumor necrosis factor alpha (TNF alpha); granulocyte monocyte colony stimulating factor (GM-CSF)
Comments
The TALL-104 line was established from the peripheral blood of a child in relapse with T-ALL.
The cells are highly cytotoxic and are capable of tumor destruction in vivo and in vitro.
IL-2 is required from optimal growth and long term cultivation, but the cells can be grown for short periods without IL-2 (growth will be slower).
The cells are positive for the alpha/beta TCR and negative for gamma/delta TCR.
Complete Growth Medium
The base medium for this cell line is RPMI-1640 (ATCC 30-2001). To make the complete growth medium, add the following components to 400 mL of the base medium:
  • Fetal Bovine Serum (ATCC 30-2020) to a final concentration of 20%.
  • 1 vial IL-2 IS (10 µg), premium grade (Miltenyi cat# 130-097744*). Contents of vial can be rehydrated with 1 mL of culture medium and then added directly to the bottle of formulated medium.
*Note: The biological activity of cat# 130-097-744 is at least 5.0 x 106 units/mg but may be as high as 9.0 x 106 units/mg per Miltenyi. This may cause the final formulation of IL-2 to be greater than 100 units/mL
Note: Complete culture medium has a recommended shelf life of one month when being stored at 2-8°C.  Vendor does not provide stability information, but suggested material should be good for up to a month after formulation.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at least 8 X 105 cells/mL.
Note: IL-2 rapidly loses its potency in medium, it is important that fresh IL-2 be used. Successful growth of this cell line is very dependent upon the quality of IL-2 used in the growth medium. ATCC recommends using the highest quality IL-2 available.
Interval: Maintain between 4 X 105 and 1 X 106 cells/mL. 
Medium Renewal: Add medium as cell density increases
Cryopreservation
Freeze medium: RPMI-1640 + 20% Gibco? Knockout SR (Thermofisher Scientific cat# 10828010) + 10% DMSO. Freeze at a cell density of at least 15 X 106 cells/ml
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 9,12
D16S539: 12
D5S818: 12,13
D7S820: 7,13
THO1: 7,9
TPOX: 8,9
vWA: 14,18
Name of Depositor
Wistar Institute
Deposited As
Homo sapiens
U.S. Patent Number
References

Santoli D, et al. Cytotoxic T-ALL cell lines and uses therefor. US Patent 5,272,082 dated Dec 21 1993

O'Connor R, et al. Growth factor requirements of childhood acute T-lymphoblastic leukemia: correlation between presence of chromosomal abnormalities and ability to grow permanently in vitro. Blood 77: 1534-1545, 1991. PubMed: 1706955

Cesano A, et al. Homing and progression patterns of childhood acute lymphoblastic leukemias in severe combined immunodeficiency mice. Blood 77: 2463-2474, 1991. PubMed: 2039829

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