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歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
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當前位置: 首頁 > ATCC代理 > T84.66A3.1A.1F2 [T84.66.A3.1-H-11] B165821
T84.66A3.1A.1F2 [T84.66.A3.1-H-11]
T84.66A3.1A.1F2 [T84.66.A3.1-H-11]
規格:
價格:
編號:B165821
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
T84.66A3.1A.1F2 [T84.66.A3.1-H-11]
商品貨號
B165821
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension, with some loosely adherent cells
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Carcinoma
Applications
The antibody reacts with CEA with an affinity constant of 2.6 X 10 exp10 l/m.
The antibody reacts strongly a variety of human carcinomas and moderately with human colonic polyps.
It cross-reacts weakly with granulocytes.
Storage Conditions
liquid nitrogen vapor phase
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Animals were immunized with purified CEA.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against carcinoembryonic antigen (CEA)
Cellular Products
immunoglobulin; monoclonal antibody; against carcinoembryonic antigen (CEA)
Comments
Animals were immunized with purified CEA.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with CEA with an affinity constant of 2.6 X 10 exp10 l/m.
The antibody reacts strongly a variety of human carcinomas and moderately with human colonic polyps.
It cross-reacts weakly with granulocytes.
Complete Growth Medium
Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.01 M HEPES and 0.01 mM 2-mercaptoethanol, 80%; fetal bovine serum, 20%
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp4 viable cells/ml. Maintain cell density between 1 X 10 exp4 and 2 X 10 exp5 viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Isotype
IgG1 kappa , IgG1; kappa light chain
Name of Depositor
Beckman Research Center of City of Hope
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Crawford FG, et al. Specific hybridoma cell line and monoclonal antibodies produced from such specific hybridoma cell line and method of using such monoclonal antibodies to detect carcinoembryonic antigens. US Patent 4,873,313 dated Oct 10 1989

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