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SUP-T1 [VB]

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價格:

編號:B165763

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

SUP-T1 [VB]

商品貨號

B165763

Organism

Homo sapiens, human

Cell Type

T lymphoblast

培養基

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

T-cell lymphoblastic lymphoma

Age

8 years

Gender

male

Ethnicity

Caucasian

Applications

This cell line is a suitable transfection host.

Storage Conditions

liquid nitrogen vapor phase

Karyotype

92, XXYY, -2, -2, -9, -9, the following markers are present: 2inv(2*) (p21q11), +2inv(2*), del(2*)(Q21), 2del(4)(q31q35), 2del(5)(p13p14), 2del(6)(q23q27), 2inv(14)(q11q32), t(7;9)(q34;q34), der(9)t(7;9)

Images

Derivation

This line was derived from malignant cells collected from the malignant pleural effusion of an 8 year old child with T-Cell Lymphoblastic Lymphoma.

Clinical Data

8 years

Caucasian

male

Antigen Expression

Leu-6 (CD1a) +; Leu-4 (CD3) +; Leu-3 (CD4) +; Leu-1 (CD5) +; Leu-9 (CD7) +; Leu-2a (CD8) +; OKT-10 (CD38) + RefSmith SD, et al. Monoclonal antibody and enzymatic profiles of human malignant T- lymphoid cells and derived cell lines. Cancer Res. 44: 5657-5660, 1984. PubMed: 6437672 RefHecht F, et al. Common region on chromosome 14 in T-cell leukemia and lymphoma. Science 226: 1445-1447, 1984. PubMed: 6438800 RefLifson JD, et al. AIDS retrovirus induced cytopathology: giant cell formation and involvement of CD4 antigen. Science 232: 1123-1127, 1986. PubMed: 3010463

Genes Expressed

immunoglobulin (cytoplasmic)

Cellular Products

immunoglobulin (cytoplasmic)

Comments

The cells express multiple T lineage markers.

The cells are E - rosette negative and beta 2 microglobulin positive.

The cells are negative for Leu 5 (CD2), sheep erythrocyte receptor, OKT 9 (CD71; transferrin receptor), surface immunoglobulin (sIg -), HLA DR, CALLA (CD10) and Tac (CD25).

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.

Subculturing

Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 3 x 10⁵ viable cells/mL and maintain between 2 x10⁵ and 2 x 10⁶ cells/mL.

Medium Renewal: 2 to 3 times per week

Cryopreservation

culture medium 95%; DMSO, 5%

Culture Conditions

Temperature: 37°C

Name of Depositor

SD Smith

Deposited As

Homo sapiens

References

Smith SD, et al. Clinical and biologic characterization of T-cell neoplasias with rearrangements of chromosome 7 band q34. Blood 71: 395-402, 1988. PubMed: 2962650

Baer R, et al. Fusion of an immunoglobulin variable gene and a T cell receptor constant gene in the chromosome 14 inversion associated with T cell tumors. Cell 43: 705-713, 1985. PubMed: 3935328

Reynolds TC, et al. Analysis of DNA surrounding the breakpoints of chromosomal translocations involving the beta T cell receptor gene in human lymphoblastic neoplasms. Cell 50: 107-117, 1987. PubMed: 3036364

Smith SD, et al. Monoclonal antibody and enzymatic profiles of human malignant T- lymphoid cells and derived cell lines. Cancer Res. 44: 5657-5660, 1984. PubMed: 6437672

Hecht F, et al. Common region on chromosome 14 in T-cell leukemia and lymphoma. Science 226: 1445-1447, 1984. PubMed: 6438800

Lifson JD, et al. AIDS retrovirus induced cytopathology: giant cell formation and involvement of CD4 antigen. Science 232: 1123-1127, 1986. PubMed: 3010463

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