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SU.86.86

規格:

價格:

編號:B165759

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

SU.86.86

商品貨號

B165759

Organism

Homo sapiens, human

Tissue

pancreas; derived from metastatic site: liver

培養基

Product Format

frozen

Morphology

Epithelial

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

ductal carcinoma

Age

57 years

Gender

Female

Ethnicity

Caucasian

Storage Conditions

liquid nitrogen vapor phase

Images

Derivation

This cell line was developed from a wedge biopsy of a liver metastasis of a pancreatic ductal carcinoma.

Clinical Data

57 years

Caucasian

female

Genes Expressed

carcinoembryonic antigen (CEA)

Tumorigenic

Yes

Effects

Yes, in nude mice inoculated subcutaneously with 10(7) cells.

(The cells produce CEA, and grow in orthotopic and heterotopic positions in nude mice within 21 days at 100% frequency (5/5).)

Comments

The cells can be lysed completely in vitro by autologous and allogeneic lymphokine activated killer (LAK) cells in the presence of interleukin 2 (IL-2). They are relatively insensitive to autologous and allogeneic natural killer (NK) cell lysis.

The cells produce CEA, and grow in orthotopic and heterotopic positions in nude mice within 21 days at 100% frequency (5/5).

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and place at 37°C to facilitate dispersal. Observe cells within 5 to 10 minutes under an inverted microscope
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
To remove trypsin-EDTA solution, transfer cell suspension to a centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes.
Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. Recommended seeding densities of 5 x 10³ to 4 x 10⁴ viable cells/cm²
Place culture vessels in incubators at 37°C. Note: subcultures can also be prepared by scraping the cells from the floor of the flask. Add fresh medium, aspirate the scraped cells to disperse them and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended by depositor

Medium Renewal: 2 to 3 times per week

Cryopreservation

Freeze medium: Culture medium ,40% ; DMSO,10% ; FBS, 50% FBS..

Storage temperature: liquid nitrogen vapor phase

STR Profile

D5S818: 7,13
D13S317: 11,12
D7S820: 8,11
D16S539: 11,13
vWA: 17
THO1: 9.3
Amelogenin: X
TPOX: 8
CSF1PO: 10,11

Name of Depositor

WD Holder

Deposited As

Homo sapiens

References

Drucker BJ, et al. A new human pancreatic carcinoma cell line produced for adoptive immunotherapy studies with lymphokine-activated killer cells in nude mice. In Vitro Cell. Dev. Biol. 24: 1179-1187, 1988. PubMed: 3264833

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