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STK 1
STK 1
規格:
價格:
編號:B165758
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
STK 1
商品貨號
B165758
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody does not react with polymerases beta and gamma, and does not neutralize polymerase alpha activity.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Antigen Expression
H-2db
Genes Expressed
immunoglobulin; monoclonal antibody; against human DNA polymerase alpha,H-2db
Cellular Products
immunoglobulin; monoclonal antibody; against human DNA polymerase alpha
Comments
Mice were immunized with fraction VIII human DNA polymerase alpha.
Spleen cells were fused with NS-1 myeloma cells.
The antibody does not react with polymerases beta and gamma, and does not neutralize polymerase alpha activity.
The ampules are labeled SDK-043.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 1 X 10 exp5 viable cells/ml and maintain at or below 1 X 10 exp6 cells/ml.
Cryopreservation
Complete growth medium supplemented with 5% (v/v) DMSO.
Isotype
IgG1
Name of Depositor
D Korn
Deposited As
mouse (B cell); mouse (myeloma)
References

Hu SZ, et al. Preparation and preliminary characterization of monoclonal antibodies against human DNA polymerase alpha. J. Biol. Chem. 257: 8386-8390, 1982. PubMed: 7085672

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