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SNU-5
SNU-5
規格:
價格:
編號:B165741
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
SNU-5
商品貨號
B165741
Organism
Homo sapiens, human
Tissue
stomach; derived from metastatic site: ascites
培養基
Product Format
frozen
Morphology
epithelial
Culture Properties
suspension, multicell aggregates
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
gastric carcinoma
Age
33 years
Gender
female
Ethnicity
Asian
Storage Conditions
liquid nitrogen vapor phase
Karyotype
This is a hypotetraploid human cell line with the modal chromosome number of 87 and 88 occurring in a total of 36% of cells. Cells having higher ploidies were found at 9.6%. Eighteen or more marker chromosomes were found in most cells, including paired der(1)t(1;21) (p36.3;q11.2); der(1)t(1;?) (p32;?); 7q+'s; del(6) (q23); 12q+; and i(17q); triple der(2)t(2;?13) (p25.3;q14.3); and other single markers. There are two kinds of 7q+'s: der(7)t(7;?) (q22.3;?) and der (7)t(7;?) (q32;?). Multiple copies of DMs were also found in most cells. Normal N1 was absent. N5, N7, N10, N19 and N20 had four copies in most cells. At least one normal X chromosome was detected, but the normal Y was absent.
Derivation
SNU-5 was line derived in 1987 by J. Park and associates from ascites of a patient with poorly differentiated carcinoma of the stomach. The patient had previously received chemotherapy including 5-fluorouracil, doxorubicin and mitomycin-C.
Derived from metastatic site, ascites
Clinical Data
33 years
Asian
female
SNU-5 was line derived in 1987 by J. Park and associates from ascites of a patient with poorly differentiated carcinoma of the stomach.
The patient had previously received chemotherapy including 5-fluorouracil, doxorubicin and mitomycin-C. The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG-72. The cells are L-dopa decarboxylase (DDC) positive.
Antigen Expression
Blood Type O; Rh +
The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG 72.
Receptor Expression
acetylcholine, muscarinic, expressed
vasoactive intestinal peptide (VIP), expressed
vasoactive intestinal peptide (VIP); acetylcholine, muscarinic
Oncogene
myc +; erb B2 +
Effects
Yes, the cells have a reported colony forming efficiency of 27% in semisolid medium.
Comments
SNU-5 cells were positive for VIP receptors but lacked gastrin receptors. The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG-72. The cells are L-dopa decarboxylase (DDC) positive.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases.These cells tend to grow in multi-cell aggregates that may lose viability when the clusters are broken or dispersed. Therefore cell counts and viabilities assays should not be performed.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 8,9
D16S539: 13
D5S818: 10
D7S820: 8,12
THO1: 9
TPOX: 11
vWA: 15,16
Population Doubling Time
34 hrs
Name of Depositor
J Park
Deposited As
Homo sapiens
Year of Origin
1987
References

Park JG, et al. Characteristics of cell lines established from human gastric carcinoma. Cancer Res. 50: 2773-2780, 1990. PubMed: 2158397

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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