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SNU-387

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價格:

編號:B165736

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

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甘油

平板

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產品名稱

SNU-387

商品貨號

B165736

Organism

Homo sapiens, human

Tissue

liver

培養基

Product Format

frozen

Morphology

epithelial

Culture Properties

adherent

Biosafety Level

2 [Cells contain Hepatitis B virus]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

grade IV/V, pleomorphic hepatocellular carcinoma

Age

41 years

Gender

female

Ethnicity

Asian

Storage Conditions

liquid nitrogen vapor phase

Karyotype

aneuploid; modal number = 67

Derivation

SNU-387 was derived in 1990 by J.-G. Park and associates from a primary hepatocellular carcinoma taken from a Korean patient who had been treated by transcatheter arterial embolization with lipoidol plus a combination of doxorubicin and mitomycin-C.

Tumor cells were initially cultured in ACL-4 medium supplemented with 5% heat-inactivated fetal bovine serum. After establishment, cultures were maintained in RPMI 1640 supplemented with 10% heat inactivated fetal bovine serum.

Clinical Data

41 years

Asian

female

Antigen Expression

Blood Type O; Rh +

Comments

Grossly, the original tumor was single nodular.

Histologically, it was predominantly compact and minor trabecular type.

The cultured cells contain a single nucleus.

Hepatitis B virus (HBV) DNA was detected by Southern blot hybridization.

HBV genomic RNA was not expressed.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.

Subculturing

Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C

Subculture Ratio: 1:3 to 1:6

Medium Renewal: Every 2 to 3 days.

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C

STR Profile

Amelogenin: X

CSF1PO: 13,14

D13S317: 12

D16S539: 9

D5S818: 10,12

D7S820: 8,10

THO1: 7

TPOX: 8,9

vWA: 14,16

Population Doubling Time

61 hrs

Name of Depositor

J Park

Deposited As

Homo sapiens

Product Origin

http://prod-cms.atcc.org/sitecore/shell/Applications/Content%20editor.aspx#

Year of Origin

1990

References

Park JG, et al. Characterization of cell lines established from human hepatocellular carcinoma. Int. J. Cancer 62: 276-282, 1995. PubMed: 7543080

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