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SML, clone 4D8

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編號:B165731

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

斜面

甘油

平板

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產品名稱

SML, clone 4D8

商品貨號

B165731

Organism

Saimiri boliviensis boliviensis, monkey, bolivian squirrel

Cell Type

B lymphoblast; Epstein-Barr virus (EBV) transforme

培養基

Product Format

frozen

Morphology

lymphoblast

Culture Properties

The cells grow in loose clumps., suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Gender

male

Applications

SML, clone 4D8 is a B lymphocyte cell line established in 1995 by transformation of mononuclear cells from a male squirrel monkey with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612).

Storage Conditions

liquid nitrogen vapor phase

Karyotype

The SML, clone 4D8 cells exhibit a karyotype consistent with the subspecies Saimiri boliviensis boliviensis; the chromosomal count was 44 with an XY sex chromosome constitution., G-banding revealed a pattern of the Bolivian karyomorph for the subspecies Saimiri boliviensis boliviensis with a submetacentric chromosomal pair 15 and an acrocentric chromosomal pair 16 resulting in six pairs of autosomal acrocentric chromosomes., The karyotype was heterozygous for the C-band polymorphism in the short arm of chromosome 14 and lacked the chromosome 5 C-band positive polymorphism found in Guyanese animals, Saimiri sciureus sciureus.

Derivation

SML, clone 4D8 is a B lymphocyte cell line established in 1995 by transformation of mononuclear cells from a male squirrel monkey with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612).

Clinical Data

SML, clone 4D8 is a B lymphocyte cell line established in 1995 by transformation of mononuclear cells from a male squirrel monkey with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612).

male

Antigen Expression

CD20 +

Genes Expressed

CD20 +

Comments

SML, clone 4D8 is a B lymphocyte cell line established in 1995 by transformation of mononuclear cells from a male squirrel monkey with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612).

The resulting cells were cloned by limiting dilution.

CITES Permit

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.

Subculturing

Cultures can be maintained by addition or replacement of medium. When replacing media, centrifuge cells and resuspend cell pellet in fresh medium at 5 x 10⁵ viable cells/mL. Maintain cultures at cell concentrations between 5 x 10⁵ and 2 x 10⁶ viable cells/mL.

Medium Renewal: Two to three times weekly

Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions

Temperature: 37°C

Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%

Name of Depositor

JG Scammell

Year of Origin

1995

References

Reynolds PD, et al. Cloning and expression of the glucocorticoid receptor from the squirrel monkey (Saimiri boliviensis boliviensis), a glucocorticoid-resistant primate. J. Clin. Endocrinol. Metab. 82: 465-472, 1997. PubMed: 9024238

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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