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SM27-1045
SM27-1045
規(guī)格:
價(jià)格:
編號:B165730
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
SM27-1045
商品貨號
B165730
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Product Format
frozen
培養(yǎng)基
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain: C57BL/6 (B cell); BALB/c (myeloma)

Applications
The antibody reacts with a unique cell marker (EM10) that is expressed on stem and progenitor cells, while being less accessible or absent on more mature cells.
Storage Conditions
liquid nitrogen vapor phase
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against EM10
Cellular Products
immunoglobulin; monoclonal antibody; against EM10
Comments
Animals were immunized with CD34 positive, CD38 negative human bone marrow cells.
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
The antibody reacts with a unique cell marker (EM10) that is expressed on stem and progenitor cells, while being less accessible or absent on more mature cells.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Iscove's modified Dulbecco's medium, 75%; fetal bovine serum, 20%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Isotype
IgM; kappa light chain
Name of Depositor
SyStemix, Inc.
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Hill BL, et al. Methods of obtaining compositions enriched for hematopoietic stem cells, antibodies for use therein, compositions derived therefrom and methods of use thereof. US Patent 5,814,440 dated Sep 29 1998

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