熟妇高潮精品一区二区三区,插少妇视频,在线免费看黄网站

SK-MEL-31

規格:

價格:

編號:B165710

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

加入購物車

在線咨詢收藏

產品名稱

SK-MEL-31

商品貨號

B165710

Organism

Homo sapiens, human

Tissue

skin

培養基

Cell Type

epithelial cell

Product Format

frozen

Morphology

epithelial

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

Malignant melanoma

Gender

female

Storage Conditions

liquid nitrogen vapor phase

Karyotype

modal number = 82; range = 74 to 84.
This is a hypotetra-/hypertriploid human cell line. The modal chromosome number is 82 occurring at 16% with polyploidy at 10.3%. The markers del(1)(q23), t(1q10p), del(7)(p13), t(7p18q), i(21q) and 5 others are common to all cells. They are all paired in most cells. N1 and N18 are absent, N11 is single-copied, and N6, N7, N8 and N16 are generally 4-copied (many cells also had 5 copies each for N7 and N8). The X is paired.

Derivation

This is one of a very extensive series of melanoma lines (see also ATCC HTB-70, ATCC HTB-71 and ATCC HTB-72) isolated by T. Takahashi and associates.

Clinical Data

female

Antigen Expression

Antigen expression: HLA A3,A29, Bw35,B40, Cw3,Cw4, DRw4

Tumorigenic

Yes

Effects

Yes, in nude mice; produces malignant melanoma

Complete Growth Medium

Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 85%; fetal bovine serum, 15%

Subculturing

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Cryopreservation

Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C

STR Profile

Amelogenin: X

CSF1PO: 10

D13S317: 12

D16S539: 13

D5S818: 11,13

D7S820: 10

THO1: 6,9

TPOX: 8,11

vWA: 16,18

Isoenzymes

AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1-2

PGM1, 1

PGM3, 1

Name of Depositor

T Takahashi

Deposited As

Homo sapiens

References

Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212

Carey TE, et al. Cell surface antigens of human malignant melanoma: mixed hemadsorption assays for humoral immunity to cultured autologous melanoma cells. Proc. Natl. Acad. Sci. USA 73: 3278-3282, 1976. PubMed: 1067619

Guldberg P, et al. Disruption of the MMAC1/PTEN gene by deletion or mutation is a frequent event in malignant melanoma. Cancer Res. 57: 3660-3663, 1997. PubMed: 9288767

王經理

手機號：17280875617

QQ：1438578920
胡經理

手機號：13345964880

QQ：2438244627
周經理

手機號：17757487661

QQ：1296385441
于經理

手機號：18067160830

QQ：2088210172
沈經理

手機號：19548299266

QQ：2662369050
李經理

手機號：13626845108

QQ：972239479