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SK-CO-1
SK-CO-1
規(guī)格:
價(jià)格:
編號(hào):B165700
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
SK-CO-1
商品貨號(hào)
B165700
Organism
Homo sapiens, human
Tissue
colon; derived from metastatic site: ascites
培養(yǎng)基
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
colorectal adenocarcinoma
Age
65 years
Gender
male
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Karyotype
(P7) hypertriploid to hypotetraploid with abnormalities including dicentrics, minutes, rings, secondary constrictions, and 8 large submetacentric markers
Clinical Data
65 years
Caucasian
male
Antigen Expression

Blood Type O; Rh+; HLA A1, A3, B7, B13

Oncogene
myc +; ras +; myb +; fos +; sis +; p53 +; abl -; ros -; src -
Genes Expressed
myc +; ras +; myb +; fos +; sis +; p53 +; abl -; ros -; src -
Comments
Ultrastructural features include microvilli, microfilaments, small mitochondria with dark granules and dilated cristae, sparse RER, few Golgi, lipid droplets, multilamellar bodies, no virus particles were detected.
The line is positive for expression of c-myc, N-myc, K-ras, H-ras, N-ras, myb, sis and fos oncogenes.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 13
D13S317: 14
D16S539: 13
D5S818: 9,11
D7S820: 8,10
THO1: 8,9
TPOX: 8,11
vWA: 18,19
Isoenzymes
AK-1, 1-2
ES-D, 1
G6PD, B
GLO-I, 1-2
Me-2, 1
PGM1, 1
PGM3, 1-2
Name of Depositor
G Trempe, LJ Old
Deposited As
Homo sapiens
References

Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975.

Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874

Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212

Tsao H, et al. Novel mutations in the p16/CDKN2A binding region of the Cyclin-dependent Kinase-4 gene. Cancer Res. 58: 109-113, 1998. PubMed: 9426066

Kutchera W, et al. Protaglandin H synthase 2 is expressed abnormally in human colon cancer: evidence for a transcriptional effect. Proc. Natl. Acad. Sci. USA 93: 4816-4820, 1996. PubMed: 8643486

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