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SK-BR-3 [SKBR3]

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價格:

編號:B165699

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

斜面

甘油

平板

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產品名稱

SK-BR-3 [SKBR3]

商品貨號

B165699

Organism

Homo sapiens, human

Tissue

mammary gland/breast; derived from metastatic site: pleural effusion

培養基

Product Format

frozen

Morphology

epithelial

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

adenocarcinoma

Age

43 years

Gender

female

Ethnicity

Caucasian

Applications

This cell line is suitable as a transfection host.

Storage Conditions

liquid nitrogen vapor temperature

Karyotype

This is a hypertriploid human cell line with the modal chromosome number of 84, occurring in 34% of cells. Cells having 80 chromosomes also occurred at a high rate (28%); the higher ploidy cells occurred at 7.3%. This cell line has a very complex chromosome composition. Thirty-five to 40% of chromosomes in a cell complement with a modal chromosome number of 84 consisted of structurally altered marker chromosomes. Several markers are longer than chromosome N1. The origins of most of these markers, however, are not clear. Some markers may have at least three individual chromosome segments. The markers [i.e., ?der(1)t(1;21) (p13;q21) [or ?t(1q21q)], ?del(2) (q13), and t(7pter--cen--?), present in some cells only] were the only ones in which portions of chromosome segments could be identified. Most cells had about three normal X chromosomes and five or more N7. The structurally normal N1, N14 and N17 were generally absent.

Images

Derivation

This cell line was derived by G. Trempe and L. J. Old in 1970 from pleural effusion cells of a patient, a White, Caucasian female, age 43, blood type A+, who had been treated with radiation, steroids, cytoxan and 5-fluorouracil.

Clinical Data

43 years

Caucasian

female

Antigen Expression

Blood Type A; Rh+; HLA A11, Bw22(+/-), B40, B18

Tumorigenic

Yes

Effects

Yes, in nude mice; forms poorly differentiated adenocarcinoma

Comments

The patient, a White, Caucasian female, age 43, blood type A+, had been treated with radiation, steroids, cytoxan and 5-fluorouracil.

No virus particles.

Ultrastructural features include microvilli and desmosomes, glycogen granules, large lysosomes, bundles of cytoplasmic fibrils.

The SK-BR-3 cell line overexpresses the HER2/c-erb-2 gene product.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Volumes are given for a 75 cm²flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Corning^® T-75 flasks (catalog #430641) are recommended for subculturing this product.

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin, 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended

Medium Renewal: 2 to 3 times per week

Cryopreservation

Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

Temperature: 37°C

STR Profile

Amelogenin: X

CSF1PO: 12

D13S317: 11,12

D16S539: 9

D5S818: 9,12

D7S820: 9,12

THO1: 8,9

TPOX: 8,11

vWA: 17

Isoenzymes

AK-1, 1-2

ES-D, 1

G6PD, B

GLO-I, 2

PGM1, 1-2

PGM3, 1

Name of Depositor

G Trempe, LJ Old

Deposited As

Homo sapiens

Year of Origin

1970

References

Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975.

Trempe GL. Human breast cancer in culture. Recent Results Cancer Res. 57: 33-41 , 1976. PubMed: 1013510

Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212

Hudziak RM, et al. Monoclonal antibodies directed to the Her2 receptor. US Patent 5,677,171 dated Oct 14 1997

Littlewood-Evans AJ, et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997. PubMed: 9393764

Chavany C, et al. p185erbB2 binds to GRP94 in vivo. J. Biol. Chem. 271: 4974-4977, 1996. PubMed: 8617772

B195598：Human genomic DNA (SK-BR-3)

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