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當(dāng)前位置: 首頁 > ATCC代理 > SHM-D33 B165691
SHM-D33
SHM-D33
規(guī)格:
價格:
編號:B165691
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
SHM-D33
商品貨號
B165691
Organism
Homo sapiens; Mus musculus, human; mouse
Tissue
heteromyeloma
培養(yǎng)基
Cell Type
B lymphoblast; somatic cell hybridoma
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Storage Conditions
liquid nitrogen vapor phase
Images
Derivation
This line was produced by fusing the human myeloma cell line FU-266, clone E-1 (HAT sensitive, 8-azaguanine resistant and resistant to G-418 - an antibiotic similar to gentamicin) with the murine myeloma P3X63Ag8.653 (see ATCC CRL-1580).
Comments
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium

HybriCare Medium (ATCC® No. 46X) supplemented with 20% high quality FBS (ATCC® 30-2020) and 0.2 mg/mL G418/Geneticin (Gibco 10131).

Subculturing
Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 4 x 104 viable cells/mL. Maintain cultures at a cell concentraton between 3 x 104 and 8 x 105 cells/mL.
Medium Renewal: Every 2 to 3 days
Note: The cells are grown in 200 to 400 mcg/mL of G-418 to maintain selection for the human chromosomes.
Cryopreservation
Complete growth medium supplemented with 7.5% (v/v) DMSO
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Population Doubling Time
36 hours
Name of Depositor
HS Kaplan
Deposited As
Homo sapiens; Mus musculus
References

Teng NN, et al. Construction and testing of mouse-human heteromyelomas for human monoclonal antibody production. Proc. Natl. Acad. Sci. USA 80: 7308-7312, 1983. PubMed: 6316357

Kearney JF, et al. A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines. J. Immunol. 123: 1548-1550, 1979. PubMed: 113458

Nilsson K, et al. Established immunoglobulin producing myeloma (IgE) and lymphoblastoid (IgG) cell lines from an IgE myeloma patient. Clin. Exp. Immunol. 7: 477-489, 1970. PubMed: 4097745

Yamagishi S, et al. Advanced glycation end products-driven angiogenesis in vitro. Induction of the growth and tube formation of human microvascular endothelial cells through autocrine vascular endothelial growth factor. J. Biol. Chem. 272: 8723-8730, 1997. PubMed: 9079706

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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