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SFR8-B6
SFR8-B6
規(guī)格:
價(jià)格:
編號(hào):B165685
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
SFR8-B6
商品貨號(hào)
B165685
Organism
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Tested and found negative for ectromelia virus (mousepox).
Derivation
Animals were immunized with partially purified glycoprotein preparations from detergent solubilized membranes from the JY human lymphoblastoid cell line.
Popliteal lymph node cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against HLA Bw6
Cellular Products
immunoglobulin; monoclonal antibody; against HLA Bw6
Comments
Animals were immunized with partially purified glycoprotein preparations from detergent solubilized membranes from the JY human lymphoblastoid cell line.
Popliteal lymph node cells were fused with Sp2/0-Ag14 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
    • Subculturing
    Medium Renewal: Every 2 to 3 days
    Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
    Culture Conditions
    Temperature: 37.0°C
    Isotype
    IgG2b
    Name of Depositor
    SF Radka
    Deposited As
    rat (B cell); mouse (myeloma)
    References

    Radka SF, et al. A monoclonal antibody directed against the HLA-BW6 epitope. J. Immunol. 128: 2804-2806, 1982. PubMed: 6176653

    White CA, et al. Constitutive transduction of peptide transporter and HLA genes restores antigen processing function and cytotoxic T cell-mediated immune recognition of human melanoma cells. Int. J. Cancer 75: 590-595, 1998. PubMed: 9466661

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