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SCaBER

規(guī)格:

價格:

編號:B165673

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

SCaBER

商品貨號

B165673

Organism

Homo sapiens, human

Tissue

urinary bladder

培養(yǎng)基

MCM-0203：SCaBER細胞專用培養(yǎng)基

Product Format

frozen

Morphology

epithelial

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

squamous cell carcinoma

Age

58 years

Gender

male

Ethnicity

Black

Karyotype

2n = 46. The stemline chromosome number is hypotriploid with the 2S component occurring at 9.2%., Eleven markers [t(1;?) del (1)(p13), t(3;?), i(4q), t(8;?), t(9;?), M6, t(1;11), t(21;22), M13, and t(20;?)] and 1 to 3 small chromosome fragments were common to most S metaphases., At least one Y chromosome was always detectable. Number 3 was nullisomic. Neither homogenously staining regions (HSR) nor double minutes (DM) were seen.

Clinical Data

58 years

Black

male

Tumorigenic

Yes

Effects

Yes, epidermoid carcinoma forms in nude mice

Comments

Contains the unusual type A isoenzyme of glucose-6-phosphate dehydrogenase.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10% This medium is formulated for use with a 5% CO₂ in air atmosphere. ATCC tested fetal bovine serum is available as ATCC? Catalog No. 30-2020 (500 mL) and ATCC? Catalog No. 30-2021 (100 mL).

Subculturing

Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution, remove trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes).

Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Cryopreservation

Culture medium, 95%; DMSO, 5%

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

Temperature: 37°C

STR Profile

Amelogenin: X,Y

CSF1PO: 11,13

D13S317: 11,12

D16S539: 11,12

D5S818: 12,13

D7S820: 8,10

THO1: 7,9

TPOX: 9,11

vWA: 16

Isoenzymes

AK-1, 1

ES-D, 1

G6PD, A

GLO-I, 1-2

Me-2, 2

PGM1, 1-2

PGM3, 1-2

Name of Depositor

C O'Toole

References

O'Toole CHuman bladder cancer lines: HLA Class I and Class II antigen expression and susceptibility to cytostatic and cytotoxic effects in vitroIn: O'Toole CIn vitro models for cancer researchvol. IVBoca Raton, FLCRC Presspp. 103-125.

O'Toole C, et al. A cell line (SCABER) derived from squamous cell carcinoma of the human urinary bladder. Int. J. Cancer 17: 707-714, 1976. PubMed: 947851

Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047

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