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SC-71
SC-71
規格:
價格:
編號:B165671
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
SC-71
商品貨號
B165671
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain:? BALB/c (B cell); BALB/c (myeloma)

Applications
The antibody reacts with type 2A myosin heavy chain in Western blots, and type IIA rat muscle fibres by immunofluorescence.
Storage Conditions
liquid nitrogen vapor phase
Derivation
Spleen cells were fused with NSO myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against rat type 2A myosin heavy chain
Cellular Products
immunoglobulin; monoclonal antibody; against rat type 2A myosin heavy chain
Comments
Animals were immunized with bovine fetal skeletal muscle myosin.
Spleen cells were fused with NSO myeloma cells.
The antibody reacts with type 2A myosin heavy chain in Western blots, and type IIA rat muscle fibres by immunofluorescence.
The cells were deposited on behalf of Dr. Schiaffino by Regeneron Pharmaceuticals, Inc.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Isotype
IgG1
Name of Depositor
S Schiaffino
Deposited As
mouse (B cell); mouse (myeloma)
References

Bottinelli R, et al. Force-velocity relations and myosin heavy chain isoform compositions of skinned fibres from rat skeletal muscle. J. Physiol. 437: 655-672, 1991. PubMed: 1890654

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