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SA22
SA22
規(guī)格:
價格:
編號:B165660
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
SA22
商品貨號
B165660
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with rboIL-1 beta and cross-reacts with its human analog (rHuIL-1 beta).
The antibodies neutralizes IL-1 activity (both macrophage derived IL-1 and recombinant IL-1 beta) in a thymocyte costimulation assay.
Derivation
Animals were immunized with purified rboIL-1 beta.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibodies neutralizes IL-1 activity (both macrophage derived IL-1 and recombinant IL-1 beta) in a thymocyte costimulation assay.
Genes Expressed
immunoglobulin; monoclonal antibody; against bovine recombinant interleukin 1 beta (rboIL-1 beta)
Cellular Products
immunoglobulin; monoclonal antibody; against bovine recombinant interleukin 1 beta (rboIL-1 beta)
Tumorigenic
Yes
Effects
Yes, forms ascites in pristane primed BALB/c mice
Comments
Animals were immunized with purified rboIL-1 beta.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with rboIL-1 beta and cross-reacts with its human analog (rHuIL-1 beta).
It does not react with other related monokines tested (ie.g., human IL-6 and TNF alpha).
The antibodies neutralizes IL-1 activity (both macrophage derived IL-1 and recombinant IL-1 beta) in a thymocyte costimulation assay.
Complete Growth Medium
Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 1 mM non-essential amino acids, 0.1 mM hypoxanthine, 0.032 mM thymidine and BME vitamins, 70%; hybridoma cloning factor (Origen), 10%; fetal bovine serum, 20%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG1
Name of Depositor
F Blecha
Deposited As
mouse (B cell); mouse (myeloma)
References

Reddy DN, et al. Isolation and characterization of monoclonal antibodies to recombinant bovine interleukin-1 beta. Vet. Immunol. Immunopathol. 36: 17-29, 1993. PubMed: 7680508

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