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S1E4 [1E4]
S1E4 [1E4]
規格:
價格:
編號:B165646
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
S1E4 [1E4]
商品貨號
B165646
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain:? BALB/c (B cell); BALB/c (myeloma)

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with BALB/c myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Eimeria tenella sporozoites
Cellular Products
immunoglobulin; monoclonal antibody; against Eimeria tenella sporozoites
Comments
Animals were immunized with E. tenella sporozoites.
Spleen cells were fused with BALB/c myeloma cells.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype
IgG1
Name of Depositor
American Cyanamid Co.
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Wong RB, et al. Antigens and monoclonal antibodies reactive against sporozoites of Eimeria spp.. US Patent 4,710,377 dated Dec 1 1987

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