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S1A(Thy-1-b)
S1A(Thy-1-b)
規格:
價格:
編號:B165644
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
S1A(Thy-1-b)
商品貨號
B165644
Organism
Mus musculus, mouse
Cell Type
T lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
lymphoma
Strain
BALB/c
Applications
This line is a Class B Thy-1 negative (Thy-1-) mutant derived from S1A.TB.4.8.2 (ATCC TIB-27).
The line may be useful in studies of the synthesis and assembly of glycophospholipid linked membrane proteins.
The cells are resistant to 0.1 mM 5-bromo-2'-deoxyuridine.
Derivation
This line is a Class B Thy-1 negative (Thy-1-) mutant derived from S1A.TB.4.8.2 (ATCC TIB-27).
Genes Expressed
The cells secrete a mutant Thy-1 glycoprotein.
Cellular Products
The cells secrete a mutant Thy-1 glycoprotein.
Comments
This line is a Class B Thy-1 negative (Thy-1-) mutant derived from S1A.TB.4.8.2 (ATCC TIB-27).
The mutation affects the biosynthesis and/or addition of the glycophospholipid anchor.
The line may be useful in studies of the synthesis and assembly of glycophospholipid linked membrane proteins.
The cells are resistant to 0.1 mM 5-bromo-2'-deoxyuridine.
Complete Growth Medium
Dulbecco's modified Eagle's medium, 90%; heat-inactivated horse serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Name of Depositor
R Hyman
Deposited As
Mus musculus
References

Conzelmann A, et al. No glycolipid anchors are added to Thy-1 glycoprotein in Thy-1-negative mutant thymoma cells of four different complementation classes. Mol. Cell. Biol. 8: 674-678, 1988. PubMed: 2895421

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