Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
male
52 years
The cells are positive for keratin by immunoperoxidase staining.
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
NOTE: Cells attach in clusters. Cells will pile and the culture does not get 100% confluent.
Moore GE, Sandberg AA. Studies of a human tumor cell line with a diploid karyotype. Cancer 17: 170-175, 1964. PubMed: 14123677
Moorhead PS. Human tumor cell line with a quasi-diploid karyotype (RPMI 2650). Exp. Cell Res. 39: 190-196, 1965. PubMed: 5831238
