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RLSD06 [Anti-PA 1, DB90 5D5.4.3]
RLSD06 [Anti-PA 1, DB90 5D5.4.3]
規(guī)格:
價格:
編號:B165610
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱
RLSD06 [Anti-PA 1, DB90 5D5.4.3]
商品貨號
B165610
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Animals were immunized with purified PA from human semen.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human prostate antigen (PA, 33000 dalton glycoprotein)
Cellular Products
immunoglobulin; monoclonal antibody; against human prostate antigen (PA, 33000 dalton glycoprotein)
Tumorigenic
Yes
Effects
Yes, forms ascites in BALB/c mice
Comments
Animals were immunized with purified PA from human semen.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Complete Growth Medium
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water and supplemented with 1.5 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
fetal bovine serum, 90%; DMSO, 10%
Isotype
IgG1
Name of Depositor
Cetus Corp.
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

M'Timkulu T, et al. Process for simultaneously detecting multiple antigens using dual sandwich immunometric assay. US Patent 4,690,890 dated Sep 1 1987

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