Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Unlike the parental line, RAW 264.7 gamma NO(-) does not produce nitric oxide upon treatment with interferon gamma alone, but requires LPS for full activation (the iNOS promoter linked to a luciferase reporter gene is also unresponsive to IFN- alone).
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Alley EW, et al. A classical enhancer element responsive to both lipopolysaccharide and interferon-gamma augments induction of the iNOS gene in mouse macrophages. Gene 158: 247-251, 1995. PubMed: 7541763
Lorsbach RB, et al. Expression of the nitric oxide synthase gene in mouse macrophages activated for tumor cell killing. Molecular basis for the synergy between interferon-gamma and lipopolysaccharide. J. Biol. Chem. 268: 1908-1912, 1993. PubMed: 7678412
