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RA3-2C2/1
RA3-2C2/1
規(guī)格:
價(jià)格:
編號(hào):B165567
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
RA3-2C2/1
商品貨號(hào)
B165567
Organism
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Leukemia
Applications
The antibody reacts with an antigen on normal B lymphocytes, a fraction of sIg- bone marrow cells and most plasma cells.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused S194/5.XX0.BU.1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse B cell antigen (p50, 50000 dalton)
Cellular Products
immunoglobulin; monoclonal antibody; against mouse B cell antigen (p50, 50000 dalton)
Comments
Animals were immunized with the mouse cell line RAW 112.
Spleen cells were fused S194/5.XX0.BU.1 myeloma cells.
The antibody reacts with an antigen on normal B lymphocytes, a fraction of sIg- bone marrow cells and most plasma cells.
It precipitates a 50000 dalton (p50) molecule from bone marrow, spleen, thymus and Abelson leukemia virus transformed cells.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 3 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Isotype
IgM
Name of Depositor
RL Coffman
Deposited As
rat (B cell); mouse (myeloma)
References

Coffman RL, Weissman IL. A monoclonal antibody that recognizes B cells and B cell precursors in mice. J. Exp. Med. 153: 269-279, 1981. PubMed: 6787164

Rotter V, et al. Abelson murine leukemia virus-induced tumors elicit antibodies against a host cell protein, P50. J. Virol. 36: 547-555, 1980. PubMed: 6159484

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