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R1E/TL8x.1
R1E/TL8x.1
規(guī)格:
價格:
編號:B165556
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
R1E/TL8x.1
商品貨號
B165556
Organism
Mus musculus, mouse
Tissue
thymus
培養(yǎng)基
Cell Type
T lymphocyte
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
lymphoma
Strain
C58/J
Applications
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions
liquid nitrogen vapor phase
Antigen Expression
H-2k; Thy-1.2
Genes Expressed
H-2k; Thy-1.2
Comments
Unlike the parental line R1.1, these cells do not express TL antigen.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: culture medium, 92.5%; DMSO, 7.5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Name of Depositor
R Hyman
Deposited As
Mus musculus
References

Hyman R, Stallings V. Characterization of a TL- variant of a homozygous TL+ mouse lymphoma. Immunogenetics 3: 75-84, 1976.

derived from R1.1 (ATCC TIB-42).

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