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QT6
QT6
規格:
價格:
編號:B165547
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
QT6
商品貨號
B165547
Organism
Coturnix coturnix japonica, quail, Japanese
Cell Type
methylcholanthrene induced
培養基
Product Format
frozen
Morphology
fibroblast
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
fibrosarcoma
Applications
This cell line is a suitable transfection host.
Storage Conditions
liquid nitrogen vapor phase
Derivation
Derived from chemically induced tumors of Japanese quail.
Tumorigenic
Yes, in animal host.
Effects
Yes, in animal host and soft agar.
Virus Susceptibility
Rous sarcoma virus
Avian encephalomyelitis virus
Rabies virus
Marek's disease virus
Complete Growth Medium
Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 10% tryptose phosphate broth and 5% bovine calf serum
Subculturing
Resuspend cells by pipetting fresh medium across the monolayer. Cells may also be dislodged by hitting culture flask against the palm of the hand. Subculture at approximately 75% confluency. Use of 0.5% polyvinylpyrrolidone (PVP) in a trypsin-EDTA solution increases the viability when subculturing by enzymatic means. Neutralize and centrifuge to remove additive.
Note: When subcultures are prepared using pipetting or mechanical means the cells adhere lightly to the flask substrate and exhibit a tendency toward clumping. 
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Cryopreservation
Complete growth medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Name of Depositor
C Moscovici
Deposited As
Coturnix coturnix japonica
References

Moscovici C, et al. Continuous tissue culture cell lines derived from chemically induced tumors of Japanese quail. Cell 11: 95-103, 1977. PubMed: 194709

Imagawa S, et al. Negative regulation of the erythropoietin gene expression by the GATA transcription factors. Blood 89: 1430-1439, 1997. PubMed: 9028967

Martin PT, Sanes JR. Integrins mediate adhesion to agrin and modulate agrin signaling. Development 124: 3909-3917, 1997. PubMed: 9367446

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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