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PS/2
PS/2
規(guī)格:
價(jià)格:
編號(hào):B165527
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
PS/2
商品貨號(hào)
B165527
Organism
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Storage Conditions
liquid nitrogen vapor phase
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against an integrin-like cellular adhesion molecule
Cellular Products
immunoglobulin; monoclonal antibody; against an integrin-like cellular adhesion molecule
Tumorigenic
Yes
Effects
Yes, produces ascites in pristane primed CB.17.SCID mice
Comments
Animals were immunized with the mouse P815 mastocytoma cell line.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody recognizes an integrin like cellular adhesion molecule expressed on murine hematopoietic cells.
The antibody blocks adhesion of B lymphocyte precursors to fibroblast-like bone marrow stromal cells.
Complete Growth Medium
RPMI 1640 medium with 2 mM L-glutamine and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 3 to 4 days
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype
IgG2b
Name of Depositor
PW Kincade
Deposited As
rat (B cell); mouse (myeloma)
References

Miyake K, et al. A VCAM-like adhesion molecule on murine bone marrow stromal cells mediates binding of lymphocyte precursors in culture. J. Cell Biol. 114: 557-565, 1991. PubMed: 1713592

Miyake K, et al. Evidence for a role of the integrin VLA-4 in lympho-hemopoiesis. J. Exp. Med. 173: 599-607, 1991. PubMed: 1997648

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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