Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
PFSK-1 cells form colonies in soft agar, and lack contact inhibition.
They express the intermediate filament protein, nestin, and are positive for neuron specific enolase (NSE).
They lack characterisics of terminally differentiated neurons or glia.
Restriction fragment length polymorphism studies showed loss of heterozygosity for multiple loci on chromosome 17.
Neither c-myc nor N-myc is amplified or re-arranged.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: 2 times per week
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual Of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005.
Fults D, et al. Establishment and characterization of a human primitive neuroectodermal tumor cell line from the cerebral hemisphere. J. Neuropathol. Exp. Neurol. 51: 272-280, 1992. PubMed: 1316433
Cote GJ, et al. Sequence requirements for regulated RNA splicing of the human fibroblast growth factor receptor-1 alpha exon. J. Biol. Chem. 272: 1054-1060, 1997. PubMed: 8995402
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
