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Pfeiffer

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價格:

編號:B165496

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

Pfeiffer

商品貨號

B165496

Organism

Homo sapiens, human

Tissue

derived from metastatic site: pleural effusion

培養基

Cell Type

lymphoblast B lymphocyte

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

diffuse large cell lymphoma; non-Hodgkin's B cell lymphoma

Age

adult

Gender

male

Ethnicity

White

Applications

This cell line is a model system for studying non-Hodgkin lymphomas.

Karyotype

The cell line contains several chromosomal abberations including the typical t(14;18)(q32;q21) translocation of follicular lymphomas.

Derivation

The Pfeiffer cell line was established in 1992 from a patient in the leukemic phase of diffuse large cell lymphoma (DLCL) with cleaved and non-cleaved nuclei.

Clinical Data

male

White

adult

Antigen Expression

CD10; Homo sapiens

CD19; Homo sapiens

CD20; Homo sapiens

CD38; Homo sapiens

CD10 +, CD19 +, CD20 +, CD38 +, CD23 -, CD39 -

Ref

Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkin's lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273

Comments

The cells are negative for Epstein-Barr virus.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.

Subculturing

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 - 5 x 10⁵ viable cells/mL. Maintain cell density between 3 x 10⁵ and 3 x 10⁶ viable cells/mL.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).

Cryopreservation

Freeze medium: Complete growth medium 95%; DMSO, 5%

Culture Conditions

Temperature: 37°C

STR Profile

CSF1PO: 10, 11

D13S317: 11, 12

D16S539: 12

D5S818: 10, 13

D7S820: 8, 12

THO1: 9, 9.3

TPOX: 9

vWA: 17, 18

Amelogenin: XY

Isotype

IgG kappa

Population Doubling Time

24 to 30 hrs

Name of Depositor

H Ben-Bassat

Deposited As

human

Year of Origin

1992

References

Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkin's lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273

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