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Panc 08.13

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編號:B165483

品牌:Mingzhoubio

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DNA

RNA

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產品名稱

Panc 08.13

商品貨號

B165483

Organism

Homo sapiens, human

Tissue

pancreas

培養基

Cell Type

Epithelial

Product Format

frozen

Morphology

epithelial

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

adenocarcinoma

Age

85 years

Gender

male

Ethnicity

White

Storage Conditions

liquid nitrogen vapor phase

Derivation

Panc 08.13 is a pancreatic adenocarcinoma epithelial cell line derived in 1995 from a primary tumor removed from the head-of-the-pancreas of a male with pancreatic adenocarcinoma.

Clinical Data

85 years

male

White

Antigen Expression

MHC class I +; MHC class II -

Oncogene

K-ras + Ref

Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602

Genes Expressed

cytokeratins 7 and 18

Cellular Products

cytokeratins 7 and 18

Tumorigenic

Yes

Effects

Yes, forms tumors in nude or SCID mice

Comments

The cell line exhibits a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine.

The cells have a reported plating efficiency of 40%.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:

10 Units/ml human recombinant insulin
fetal bovine serum to a final concentration of 15%

Subculturing

Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53% (w/v) EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C

Subculture Ratio: 1:2 to 1:4

Medium Renewal: Every 2 to 3 days.

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994

Cryopreservation

culture medium 95%; DMSO, 5%

Culture Conditions

Temperature: 37°C

STR Profile

Amelogenin: X

CSF1PO: 11

D13S317: 13

D16S539: 13

D5S818: 13,14

D7S820: 11

THO1: 6,9.3