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PA2.1
PA2.1
規格:
價格:
編號:B165465
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
PA2.1
商品貨號
B165465
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reactive correlates with the classical HLA A2 specificity.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Animals were immunized with purified HLA A2.
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against HLA A2, Aw69
Cellular Products
immunoglobulin; monoclonal antibody; against HLA A2, Aw69
Comments
Animals were immunized with purified HLA A2.
Spleen cells were fused with NS-1 myeloma cells.
Mutation at position 107 of the alpha 2 domain of the HLA-A2 molecule results in complete loss of binding of PA2.1.
The antibody reactive correlates with the classical HLA A2 specificity.
PA2.1 and BB7.2 (ATCC HB-82) recognize overlapping epitopes on the alpha 2 domain of HLA-A2.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Modified Dulbecco's medium with 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG1
Name of Depositor
P Parham
Deposited As
mouse (B cell); mouse (myeloma)
References

Parham P, Bodmer WF. Monoclonal antibody to a human histocompatibility alloantigen, HLA-A2. Nature 276: 397-399, 1978. PubMed: 714164

Santos-Aguado J, et al. Molecular characterization of serologic recognition sites in the human HLA-A2 molecule. J. Immunol. 141: 2811-2818, 1988. PubMed: 2459229

Hogan KT, Brown SL. Localization and characterization of serologic epitopes on HLA-A2. Hum. Immunol. 33: 185-192, 1992. PubMed: 1377666

Brodsky FM, et al. Monoclonal antibodies for analysis of the HLA system. Immunol. Rev. 47: 3-61, 1979. PubMed: 95015

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