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P3X63Ag8.653
P3X63Ag8.653
規格:
價格:
編號:B165458
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
P3X63Ag8.653
商品貨號
B165458
Organism
Mus musculus, mouse
Cell Type
B lymphoblast
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
plasmacytoma; myeloma
Strain
BALB/c
Applications
This cell line is a suitable transfection host.
Storage Conditions
liquid nitrogen vapor phase
Antigen Expression
H-2d
Comments
The cells are resistant to 8-azaguanine and are HAT sensitive.
They can be used as fusion partners for producing hybridomas.
The cells have been reported to be cholesterol auxotrophs due to a deficiency in 3-ketosteroid reductase activity.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start new cultures at 4 X 105 viable cells/mL and subculture before the cell density reaches 2 X 106 cells/mL.
Medium Renewal: Every 3 to 4 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Name of Depositor
JF Kearney
Deposited As
Mus musculus
References

Kearney JF, et al. A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines. J. Immunol. 123: 1548-1550, 1979. PubMed: 113458

Sato JD, et al. Effects of proximate cholesterol precursors and steroid hormones on mouse myeloma growth in serum-free medium. In Vitro Cell. Dev. Biol. 24: 1223-1228, 1988. PubMed: 3209588

Oppermann M, et al. Monoclonal antibodies reveal receptor specificity among G-protein-coupled receptor kinases. Proc. Natl. Acad. Sci. USA 93: 7649-7654, 1996. PubMed: 8755530

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