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OM 3-1.1
OM 3-1.1
規(guī)格:
價(jià)格:
編號(hào):B165439
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
OM 3-1.1
商品貨號(hào)
B165439
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Tested and found negative for ectromelia virus (mousepox).
Derivation
Animals were immunized with purified human alpha-fetoprotein.
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human alpha fetoprotein (AFP, alpha-fetoprotein)
Cellular Products
immunoglobulin; monoclonal antibody; against human alpha fetoprotein (AFP, alpha-fetoprotein)
Comments
Animals were immunized with purified human alpha-fetoprotein.
Spleen cells were fused with NS-1 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 0.005 mM 2-mercaptoethanol and HAT, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 3 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype
IgG1; kappa light chain
Name of Depositor
JW Pickering
Deposited As
mouse (B cell); mouse (myeloma)
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