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OKT 5
OKT 5
規格:
價格:
編號:B165434
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
OKT 5
商品貨號
B165434
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
In order to detect antibody production from this line by immunodiffusion a ten fold concentration of the supernatant was required.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with P3X63Ag8.U1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human T cell (human cytotoxic/suppressor T lymphocyte)
Cellular Products
immunoglobulin; monoclonal antibody; against human T cell (human cytotoxic/suppressor T lymphocyte)
Comments
Animals were immunized with human peripheral blood T cell.
Spleen cells were fused with P3X63Ag8.U1 myeloma cells.
In order to detect antibody production from this line by immunodiffusion a ten fold concentration of the supernatant was required.
Complete Growth Medium
Iscove's modified Dulbecco's medium, 80%; fetal bovine serum, 20%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG1
Name of Depositor
Ortho Pharmaceutical Corporation
U.S. Patent Number
References

Hoffman RA, et al. Simple and rapid measurement of human T lymphocytes and their subclasses in peripheral blood. Proc. Natl. Acad. Sci. USA 77: 4914-4917, 1980. PubMed: 6968909

Kung PC, Goldstein G. Monoclonal antibody to human cytotoxic and suppressor T cells and methods of preparing same. US Patent 4,364,932 dated Dec 21 1982

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