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NCI-H841 [H841]
NCI-H841 [H841]
規(guī)格:
價(jià)格:
編號(hào):B165373
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
NCI-H841 [H841]
商品貨號(hào)
B165373
Organism
Homo sapiens, human
Tissue
lung
培養(yǎng)基
Product Format
frozen
Culture Properties
mixed: adherent and suspension, Cultures grow as floating aggregates of round cell clusters and as attached epithelial-like cells.
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
stage L,carcinoma; variant small cell lung cancer
Age
51 years
Gender
male
Ethnicity
Caucasian, White
Applications
The line was established in October 1984.
Karyotype
t(2p,3p), t(3q,9q)
Derivation
The line was established in October 1984.
Clinical Data
The patient received prior chemotherapy and radiation therapy.
The patient was a smoker.
male
Caucasian, White
51 years
Comments
The line was established in October 1984.
The patient received prior chemotherapy and radiation therapy.
The patient was a smoker.
70.
Complete Growth Medium
HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4.5 mM)
  7. 5% fetal bovine serum (final conc.)

Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended
Medium Renewal: Add fresh medium as cell density increases.
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium.
Attached cells may be subcultured using 0.25% trypsin-0.03% EDTA.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X,Y
CSF1PO: 11
D13S317: 12
D16S539: 13
D5S818: 11,12
D7S820: 10,13
THO1: 6,9
TPOX: 11,12
vWA: 16,17
Name of Depositor
AF Gazdar, JD Minna
Deposited As
Homo sapiens
Year of Origin
1984
References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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