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NCI-H774 [H774]
NCI-H774 [H774]
規(guī)格:
價(jià)格:
編號(hào):B165368
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
NCI-H774 [H774]
商品貨號(hào)
B165368
Organism
Homo sapiens, human
Tissue
lung; derived from metastatic site: soft tissue
培養(yǎng)基
Product Format
frozen
Morphology
floating aggregates of round cell clusters
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
stage E, carcinoma; classic small cell lung cancer
Age
43 years
Gender
male
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Karyotype
del(p13-p21), del(p11-pter), del(p13-pter)
Derivation
The line was established in July 1984.
Clinical Data
The patient was a smoker
male
Caucasian
43 years old
30 pack years
Comments
Fast growing.

Complete Growth Medium
HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4.5 mM)
  7. 5% fetal bovine serum (final conc.)

Subculturing
Cultures can be maintained by the addition of fresh medium or alternatively cultures can be established by centrifugation with subsequent resuspension in fresh medium. Cultures grow as floating aggregates of round cell clusters. Single cells are dead and clusters are viable.

Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density).
Cryopreservation
Culture medium, 90%; DMSO, 5%; extra FBS, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 8
D16S539: 12
D5S818: 11
D7S820: 9,11
THO1: 6,9.3
TPOX: 8
vWA: 15,17
Name of Depositor
AF Gazdar, JD Minna
Deposited As
Homo sapiens
Year of Origin
1984
References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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