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NCI-H69 [H69]
NCI-H69 [H69]
規格:
價格:
編號:B165359
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
NCI-H69 [H69]
商品貨號
B165359
Organism
Homo sapiens, human
Tissue
lung
培養基
Product Format
frozen
Morphology
floating aggregates
Culture Properties
suspension, multicell aggregates
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
carcinoma; small cell lung cancer
Age
55 years adult
Gender
male
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor temperature
Karyotype
modal number = 76 to 78; range = 40 to 87
This is an aneuploid human male cell line. Monosomy of many of the normal chromosomes is noted as well as bisomy in this subtetraploid cell line; however, translocations and deletions involving many of the missing chromosomes are noted, and these chromosomal rearrangements appear to be stable and generally paired. Twelve marker chromosomes were identified including: der(16)t(1;16)(q21;q23), der(22)t(4;22)(q12;q13), der(12)t(11;12)(q23;p12), del(17)(p11), der(19)t(5;19)(?q21;q13) and others.
Images
Clinical Data
55 years adult
Caucasian
male
Receptor Expression
insulin-like growth factor II (IGF II)
Oncogene
myc +; myb +; fes +; fms +; raf +; ras +
Genes Expressed

Oncogenes: myc +; myb +; fes +; fms +; raf +; ras +

Tumorigenic
Yes
Effects
Yes, in nude mice
Yes, forms colonies in soft agar
Comments
This cell line is aneuploid, will form colonies in soft agar and retains small cell carcinoma morphology and ultrastructure as well as APUD cell characteristics.
The cells grow in aggregates, thus cell counts are not accurate.
The cells stain positively for cytokeratins.
The line can be adapted to grow in shaker flask or spinner flask systems.
The N-myc gene is amplified, and there is expression of the mRNA and protein.
C-myc mRNA, but not protein, is expressed at a low level.
There is expression of c-myb, v-fes, v-fms, c-raf 1, Ha-ras, Ki-ras and N-ras mRNA.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Attached cells can be detached by shaking flask Allow aggregates to settle to the bottom of the flask, remove and discard the supernatant medium. Add fresh medium, disperse cells by gentle pipetting and dispense into new flasks. Do not break down aggregates. Subculture every 6 to 8 days.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 times weekly
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37.0°C
STR Profile
CSF1PO: 10, 12
D13S317: 12
D16S539: 11
D5S818: 11, 13
D7S820: 9
THO1: 8, 9
TPOX: 10
vWA: 16, 17
Amelogenin: XY
Isoenzymes
AK-1, 1
ES-D, 2
G6PD, B
GLO-I, 1-2
Me-2, 1
PGM1, 2
PGM3, 1
Name of Depositor
AF Gazdar
Deposited As
Homo sapiens
References

Little CD, et al. Amplification and expression of the c-myc oncogene in human lung cancer cell lines. Nature 306: 194-196, 1983. PubMed: 6646201

Bepler G, et al. Expression of p64c-myc and neuroendocrine properties define three subclasses of small cell lung cancer. Oncogene 4: 45-50, 1989. PubMed: 2536917

Schardt C, et al. Characterization of insulin-like growth factor II receptors in human small cell lung cancer cell lines. Exp. Cell Res. 204: 22-29, 1993. PubMed: 8380141

Broers JL, et al. Spontaneous changes in intermediate filament protein expression patterns in lung cancer cell lines. J. Cell Sci. 91: 91-108, 1988. PubMed: 2473086

Rygaard K, et al. Expression of myc family oncoproteins in small-cell lung-cancer cell lines and xenografts. Int. J. Cancer 54: 144-152, 1993. PubMed: 8386707

Gazdar AF, et al. Establishment of continuous, clonable cultures of small-cell carcinoma of lung which have amine precursor uptake and decarboxylation cell properties. Cancer Res. 40: 3502-3507, 1980. PubMed: 6108156

Adi F, et al. Establishment of Continuous, Clonable Cultures of Small-Cell Carcinoma of the Lung Which Have Amine Precursor Uptake and Decarboxylation Cell Properties. Cancer Res. 40: 3502-3507, 1980. PubMed: 6108156

Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257

Gazdar AF, et al. Characterization of variant subclasses of cell lines derived from small cell lung cancer having distinctive biochemical, morphological, and growth properties. Cancer Res. 45: 2924-2930, 1985. PubMed: 2985258

Kiefer PE, et al. Amplification and expression of protooncogenes in human small cell lung cancer cell lines. Cancer Res. 47: 6236-6242, 1987. PubMed: 2824028

Hensel CH, et al. Altered structure and expression of the human retinoblastoma susceptibility gene in small cell lung cancer. Cancer Res. 50: 3067-3072, 1990. PubMed: 2159370

Lung Cancer 4: 155-161, 1988.

Cairns P, et al. Genomic organization and mutation analysis of Hel-N1 in lung cancers with chromosome 9p21 deletions. Cancer Res. 57: 5356-5359, 1997. PubMed: 9393760

Geiger T, et al. Antitumor activity of a PKC-alpha antisense oligonucleotide in combination with standard chemotherapeutic agents against various human tumors transplanted into nude mice. Anticancer Drug Des. 13: 35-45, 1998. PubMed: 9474241

The cells form tumors with typical small cell carcinoma histology.

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