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NCI-H661 [H661]
NCI-H661 [H661]
規格:
價格:
編號:B165357
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
NCI-H661 [H661]
商品貨號
B165357
Organism
Homo sapiens, human
Tissue
lung; derived from metastatic site: lymph node
培養基
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
carcinoma; large cell lung cancer
Age
43 years
Gender
male
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Karyotype
hyperhexaploid; modal number = 142; range = 130 to 153. Each cell had over 70 marker chromosomes. Among the markers were t(1p10q), del(1)(q11/q12), 6q+, 2q+,der(20)t(12;20)(q11;q13). Some of these had 2 to copies per cell. Most cells also had 5 to 10 microchromosomes (metacentric like). Structurally normal N2,N4 and N9 were absent; two copies of the X and multiple copies of the Y were detected.
Clinical Data
43 years
Caucasian
male
Comments
The line lacks ultrastructural and biochemical evidence of squamous differentiation or mucin production.
The cells express easily detectable p53 mRNA at levels comparable to normal lung tissue, and exhibit no gross structural DNA abnormalities.
The cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA, rinse quickly and remove trypsin leaving 1 to 2 mL. Let the culture sit at room temperature (or at 37°C) until the cells detach. Add fresh medium, disperse cells and transfer to a new flask.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10
D13S317: 11
D16S539: 12
D5S818: 11
D7S820: 8,10
THO1: 8
TPOX: 8
vWA: 17
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1-2
Me-2, 0
PGM1, 1
PGM3, 1
Name of Depositor
AF Gazdar, JD Minna
Deposited As
Homo sapiens
Year of Origin
1982
References

Banks-Schlegel SP, et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. PubMed: 2578876

Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494

Levitt ML, et al. Cross-linked envelope-related markers for squamous differentiation in human lung cancer cell lines. Cancer Res. 50: 120-128, 1990. PubMed: 1967140

The NCI-H661 cell line was derived by A.F. Gazdar and H. Oie in 1982 from the lymph node of a patient with large cell cancer of the lung. The specimen was obtained prior to therapy.

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