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NCI-H1755 [H1755]
NCI-H1755 [H1755]
規(guī)格:
價格:
編號:B165283
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
NCI-H1755 [H1755]
商品貨號
B165283
Organism
Homo sapiens, human
Tissue
lung; derived from metastatic site: liver
培養(yǎng)基
Product Format
frozen
Morphology
Epithelial-like and/or rounded
Culture Properties
Adherent, with single cells and small clusters in suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
stage 4, adenocarcinoma; non-small cell lung cancer
Age
65 years
Gender
female
Ethnicity
Caucasian
Derivation
The line was established in October 1987.
Clinical Data
65 years
Caucasian
female
The patient was a smoker.
60 pack years.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing

Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium. Cultures grow as floating aggregates of round cell clusters and attached epithelial-like cells. Attached cells can be subcultured using 0.25% Trypsin-0.53 mM EDTA. Cultures may lose viability when the aggregates are broken down. Accordingly, cell counts are not performed while expanding the CRL-5892™ cell line.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:10 is recommended
Medium Renewal: Every 3 to 4 days
Cryopreservation
Complete culture medium, 50%; FBS, 40%; DMSO, 10%
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 10,12
D16S539: 11,12
D5S818: 11,12
D7S820: 12
F13A01: 4,6
F13B: 8,10
FESFPS: 11
LPL: 12
THO1: 7
TPOX: 8
vWA: 18
Name of Depositor
AF Gazdar, JD Minna
Deposited As
Homo sapiens
Year of Origin
1987
References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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