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NCI-H1618 [H1618]
NCI-H1618 [H1618]
規格:
價格:
編號:B165271
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
NCI-H1618 [H1618]
商品貨號
B165271
Organism
Homo sapiens, human
Tissue
lung; derived from metastatic site: bone marrow
培養基
Product Format
frozen
Culture Properties
suspension, multicell aggregates
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
stage E,carcinoma; small cell lung cancer
Age
55 years
Gender
female
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Derivation
The line was established in April 1987.
Clinical Data
Caucasian
female
The patient was a smoker.
50 pack years.
Complete Growth Medium
HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4.5 mM)
  7. 5% fetal bovine serum (final conc.)

Subculturing
Cells can be maintained by the addition of fresh medium
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with an additional 5% fetal bovine serum and 7.5% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 9
D16S539: 11,13
D5S818: 10
D7S820: 8
THO1: 6
TPOX: 8,11
vWA: 16,17
Name of Depositor
AF Gazdar, JD Minna
Deposited As
Homo sapiens
Year of Origin
April, 1987
References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Cairns P, et al. Genomic organization and mutation analysis of Hel-N1 in lung cancers with chromosome 9p21 deletions. Cancer Res. 57: 5356-5359, 1997. PubMed: 9393760

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