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NCI-H1238 [H1238]
NCI-H1238 [H1238]
規格:
價格:
編號:B165254
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
NCI-H1238 [H1238]
商品貨號
B165254
Organism
Homo sapiens, human
Product Format
frozen
培養基
Culture Properties
suspension; some adherent cells
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
stage E, carcinoma; small cell lung cancer (SCLC)
Age
64 years
Gender
male
Ethnicity
White
Derivation
The line was established in November 1985.
Clinical Data
The patient was a smoker.
male
White
64 years
Comments
The line was established in November 1985.
The patient was a smoker.
130 pack years.
Complete Growth Medium
HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4.5 mM)
  7. 5% fetal bovine serum (final conc.)

Subculturing
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)
Cultures can be maintained by addition of fresh medium.
Cells grow as floating aggregates of round cell clusters.
Some cells will attach.
Cryopreservation
Culture medium with an additional 45% fetal bovine serum, 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 11
D13S317: 8,14
D16S539: 10
D5S818: 13
D7S820: 11
THO1: 9,9.3
TPOX: 8
vWA: 18,19
Name of Depositor
AF Gazdar, JD Minna
Deposited As
Homo sapiens
Year of Origin
1985
References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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