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NAMALWA

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編號:B165224

品牌:Mingzhoubio

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RNA

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產品名稱

NAMALWA

商品貨號

B165224

Organism

Homo sapiens, human

Cell Type

B lymphocyte

培養基

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

[Cells contain Herpesvirus]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

Burkitt's lymphoma

Age

3 years

Gender

female

Applications

This cell line is a suitable transfection host.

Storage Conditions

liquid nitrogen vapor phase

Karyotype

This is a human cell line with the hypodiploid chromosome count. The modal chromosome number was 44, occurring in 30% of cells. The rate of cells with higher ploidies was 2.8%. Twelve to 14 marker chromosomes were common to most cells. Among these were ins(1;?) (q11;?), ins(3) (q11q29), del(3) (p12/13), der(6)t(3;6) (p21;p25), t(15q21q) and seven to nine others. Normal N3 was absent, and N7 had three copies per cell. The X chromosome was single. There was no Y chromosome.

Images

Clinical Data

female

3 years

Genes Expressed

immunoglobulin

Comments

The cells contain the Epstein-Barr virus (EBV) genome.

Complete Growth Medium

RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 92.5%; fetal bovine serum, 7.5%

Subculturing

Cultures can be maintained by addition or replacement of fresh medium. Cultures should be started at 5 x 10⁵ viable cells/mL and subcultured at 2 x 10⁶ cells/mL.

Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 3 days

Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C

STR Profile

Amelogenin: X

CSF1PO: 10,11

D13S317: 11,12

D16S539: 9

D5S818: 12,13

D7S820: 11

THO1: 7,9.3

TPOX: 6,11

vWA: 14

Isotype

IgM; lambda light chain

Name of Depositor

NB Finter

Deposited As

Homo sapiens

References

Klein F, et al. Large-scale production and concentration of human lymphoid interferon. Antimicrob. Agents Chemother. 15: 420-427, 1979. PubMed: 464569

Benjamin D, et al. Immunoglobulin secretion by cell lines derived from African and American undifferentiated lymphomas of Burkitt's and non-Burkitt's type. J. Immunol. 129: 1336-1342, 1982. PubMed: 6286763

Klein G, et al. Sensitivity of Epstein-Barr virus (EBV) producer and non-producer human lymphoblastoid cell lines to superinfection with EB-virus. Int. J. Cancer 10: 44-57, 1972. PubMed: 4122458

Nyormoi O, et al. Sensitivity to EBV superinfection and IUdR inducibility of hybrid cells formed between a sensitive and a relatively resistant Burkitt lymphoma cell line. Int. J. Cancer 12: 396-408, 1973. PubMed: 4365093

Cross References

Nucleotide (GenBank) : X59969 H.sapiens BiP/GRP78 promoter.

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