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N2.261
N2.261
規格:
價格:
編號:B165220
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
N2.261
商品貨號
B165220
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with the slow and fast IIa isoforms of human and rodent skeletal muscle myosin.
Derivation
Animals were immunized with partially purified human adult skeletal muscle myosin heavy chain.
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against slow and fast IIa human and rodent myosin heavy chain (MyHC)
Cellular Products
immunoglobulin; monoclonal antibody; against slow and fast IIa human and rodent myosin heavy chain (MyHC)
Comments
Animals were immunized with partially purified human adult skeletal muscle myosin heavy chain.
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
The antibody reacts with the slow and fast IIa isoforms of human and rodent skeletal muscle myosin.
Complete Growth Medium
Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 0.1 mM nonessential amino acids and 1 mM sodium pyruvate, 90%; iron supplemented bovine calf serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG1
Name of Depositor
HM Blau
Deposited As
mouse (B cell); mouse (myeloma)
References

Hughes SM, et al. Three slow myosin heavy chains sequentially expressed in developing mammalian skeletal muscle. Dev. Biol. 158: 183-199, 1993. PubMed: 7687223

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