Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease
Hepatoma; Novikoff hepatoma
Gender
male
Strain
Sprague-Dawley
Storage Conditions
liquid nitrogen vapor phase
Derivation
The N1-S1 cell line was established from a hepatoma induced by feeding 4-dimethylaminoazobenzene to a male rat.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 4 X 104 viable cells/mL and subculture at 1 to 1.5 X 106 cells/mL (after about 3 days). Excessive clumping of cells can often be cured by trypsinizing the cells to disperse them.
