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N1E-115
N1E-115
規(guī)格:
價格:
編號:B165217
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
N1E-115
商品貨號
B165217
Organism
Mus musculus, mouse
Tissue
brain
培養(yǎng)基
Cell Type
neuroblast
Product Format
frozen
Morphology
neuroblast
Culture Properties
loosely adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
neuroblastoma
Strain
A/J
Applications
This cell line is a suitable transfection host. The cells can be used to study neurotransmitters and their receptors with radioligand binding, second messenger synthesis and electrophysiological changes.
Storage Conditions
liquid nitrogen vapor phase
Karyotype
modal number = 192
Images
Derivation
The N1E-115 cell line was established in 1971 by T. Amano, E. Richelson, and M. Nirenberg by cloning the C-1300 spontaneous mouse neuroblastoma tumor, C-1300.
Receptor Expression
acetylcholine, muscarinic m1; acetylcholine, muscarinic m2; vasoactive intestinal peptide (VIP)
adenosine; angiotensin II; bradykinin; enkephalin; glucagon; histamine H1; 5-hydroxytryptamine (serotonin, 5HT3); neurotensin; prostaglandin E; somatostatin; thrombin
Comments

The clone N1E was subcloned by isolation of single cells on glass shards.

The adrenergic clone N1E-115 exhibits high levels of activity of the enzymes tyrosine hydroxylase and acetylcholinesterase which are necessary for neurotransmitter synthesis but are almost devoid of choline acetyltransferase.

These cells contain 14 receptors for neurotransmitters.


Complete Growth Medium
Dulbecco's modified Eagle's medium with 4.5 g/L glucose (without sodium pyruvate), 90%; fetal bovine serum, 10%
Subculturing
  1. Remove medium, rinse with Modified Puck's Saline D1 solution:
    1. Glucose                       5.5 mM
    2. KCl                             5.4 mM
    3. Sucrose                     58.4 mM
    4. Na2HPO4-7H2O         0.17 mM
    5. NaCl                          138 mM
    6. KH2PO4                     0.22 mM
    Adjusted to a pH of 7.4±0.05 with 0.1 N NaOH or 0.N HCl Adjusted to an osmomolality of 340±5 mosM with either sucrose or H20 to decrease or increase the osmomolality.
  2. Allow flasks to remain at room temperature until cells detach.
  3. To remove saline, add fresh culture medium, transfer cell suspension to centrifuge tube and spin at approximately 125 x for 5 to 10 minutes.
  4. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.
  5. Place culture vessels in incubators at 37°C
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended.
Medium Renewal: Every 2 to 3 days.

Note: If DMEM formulation contain 1.5 g/L sodium bicarbonate.

Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Population Doubling Time
36 hrs
Name of Depositor
E Richelson
Deposited As
Mus musculus
References

. Methods in neurotransmitter receptor analysis. New York: Raven Press; 1990.

Richelson E. The use of cultured cells in the study of mood-normalizing drugs. Pharmacol. Toxicol. 66 suppl.3: 69-75, 1990. PubMed: 2179933

Amano T, et al. Neurotransmitter synthesis by neuroblastoma clones (neuroblast differentiation-cell culture-choline acetyltransferase- acetylcholinesterase-tyrosine hydroxylase-axons-dendrites). Proc. Natl. Acad. Sci. USA 69: 258-263, 1972. PubMed: 4400294

Richelson E. Regulation of tyrosine hydroxylase activity in mouse neuroblastoma clone N1E-115. J. Neurochem. 21: 1139-1145, 1973. PubMed: 4148612

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