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MYB 2-37.63
MYB 2-37.63
規格:
價格:
編號:B165211
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
MYB 2-37.63
商品貨號
B165211
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against myb (v-myb and chicken c-myb) proteins
Cellular Products
immunoglobulin; monoclonal antibody; against myb (v-myb and chicken c-myb) proteins
Comments
Mice were immunized with bp37v.myb protein.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody does not block the binding of MYB 2-7.77 (ATCC CRL-1724) or MYB 2-3.76 (ATCC CRL-1728).
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG2b
Name of Depositor
J Bishop, GI Evan
Deposited As
mouse (B cell); mouse (myeloma)
References

Evan GI, et al. Isolation of monoclonal antibodies specific for products of avian oncogene myb. Mol. Cell. Biol. 4: 2843-2850, 1984. PubMed: 6084811

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