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MV-4-11

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編號:B165206

品牌:Mingzhoubio

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產品名稱

MV-4-11

商品貨號

B165206

Organism

Homo sapiens, human

Tissue

peripheral blood

培養基

Cell Type

macrophage

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

biphenotypic B myelomonocytic leukemia

Age

10 years

Gender

male

Applications

This cell line is a suitable transfection host.

Storage Conditions

liquid nitrogen vapor phase

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Karyotype

48, XY, t(4;11)(q21;q23), +8, +19

Derivation

The MV4-11 cell line was established by Rovera and associates from the blast cells of a 10-year-old male with biphenotypic B-myelomonocytic leukemia. The growth factor, granulocyte/macrophage colony-stimulating factor (GM-CSF), was required to establish this cell line and growth factors are necessary for its continuous proliferation in chemically defined medium [PubMed: 3496132].

Clinical Data

male

The MV4-11 cell line was established by Rovera and associates from the blast cells of a 10-year-old male with biphenotypic B-myelomonocytic leukemia.

Antigen Expression

CD4; Homo sapiens

CD10; Homo sapiens

CD15, human; Homo sapiens

CD4 (40-96%); CD10 (4-11%); CD15 (96-99%)

Genes Expressed

CD4; Homo sapiens, CD10; Homo sapiens, CD15, human; Homo sapiens, CD4 (40-96%); CD10 (4-11%); CD15 (96-99%)

Comments

This line can be propagated in medium supplemented with 10% FBS without the addition of growth factors. IL-3 can independently support the long-term growth of this cell line, but IL-3 antagonized the proliferation of MV4-11 cells in the presence of GM-CSF when both factors were used at very low concentrations. Granulocyte colony stimulating factor (G-CSF) synergized with GM-CSF in inducing proliferation of MV4-11 cells; G-CSF alone causes a transient stimulation of the cell line.

This line was formerly designated ATCC HTB-189.

Over 96% of these cells are positive by indirect immunofluorescence for the myelomonocytic antigen CD15, 40-96% are positive for the monocytic antigen CD4, and 4-11% are positive for CD10 [PubMed: 3500218].

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10⁵ cells/mL and maintain between 1 X 10⁵ and 1 X 10⁶ cells/mL. Corning^® T-75 flasks (catalog #431464) are recommended for subculturing this product.

Medium Renewal: Every 2 to 3 days

Note: If the cells are maintained in a serum-free medium, it is necessary to add the following: 0.005 mg/mL transferrin, 0.005 mg/mL insulin, and 5 ng/mL GM-CSF.

Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

Temperature: 37°C

STR Profile

Amelogenin: X,Y

CSF1PO: 10,12

D13S317: 13

D16S539: 11,12

D5S818: 11,12

D7S820: 8,9

THO1: 8,9.3

TPOX: 8,11

vWA: 14,15

Name of Depositor

Wistar Institute

Deposited As

Homo sapiens

U.S. Patent Number

5,516,512

References

Lange B, et al. Growth factor requirements of childhood acute leukemia: establishment of GM-CSF-dependent cell lines. Blood 70: 192-199, 1987. PubMed: 3496132

Santoli D, et al. Synergistic and antagonistic effects of recombinant human interleukin (IL) 3, IL-1 alpha, granulocyte and macrophage colony-stimulating factors (G-CSF and M-CSF) on the growth of GM-CSF-dependent leukemic cell lines. J. Immunol. 139: 3348-3354, 1987. PubMed: 3500218

If the cells are maintained in a serum-free medium, it is necessary to add the following: 0.005 mg/ml transferrin, 0.005 mg/ml insulin, and 5 ng/ml GM-CSF.

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