Comments
The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--1.3, 2.6; HindIII--2.7; PvuII--0.6, 1.6; SstI--0.4, 1.7, 2.3; SalI--1.3.
The MTKP 97-12 cell line was produced by transfecting LMTK- cells with the expression plasmid pMTp97b that contains a cDNA sequence coding for the p97 melanoma associated antigen.
The cells produce approximately 4,000,000 molecules of p97 per cell under the control of the mouse metallothionein promoter.
A metallothionein promoter has been added upstream of the cDNA sequence. Expression can be induced by treating with 80 uM ZnCl and 9 uM CdCl for 24 hrs.
Digestion with EcoRI and HindIII excises the insert in two fragments. The larger fragment contains the complete coding sequence, that extends approximately from nt 60 to nt 2300.
