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mSXL 18
mSXL 18
規格:
價格:
編號:B165201
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
mSXL 18
商品貨號
B165201
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with epitopes expressed on the female specific sex lethal gene product.
The antibody can be used to study the products of the Sxl gene, and can be a a marker for female cells in male - female chimeras.
The antibody cross-reacts with antigens present in extracts of D. melanogaster testes, HeLa cells and the common house fly (Musca domestica).
It binds Protein A and is useful for immunoprecipitation assays and tissue staining in whole mount embryo and ovaries.
Derivation
Animals were immunized with purified LacZ - Sxl fusion protein.
Spleen cells were fused with Sp2/0 myeloma cells.
Clinical Data
The antibody reacts with epitopes expressed on the female specific sex lethal gene product.
The antibody can be used to study the products of the Sxl gene, and can be a a marker for female cells in male - female chimeras.
Genes Expressed
immunoglobulin; monoclonal antibody; against the female specific sex lethal gene product of Drosophila melanogaster (Sxl)
Cellular Products
immunoglobulin; monoclonal antibody; against the female specific sex lethal gene product of Drosophila melanogaster (Sxl)
Comments
Animals were immunized with purified LacZ - Sxl fusion protein.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody reacts with epitopes expressed on the female specific sex lethal gene product.
The antibody can be used to study the products of the Sxl gene, and can be a a marker for female cells in male - female chimeras.
The antibody cross-reacts with antigens present in extracts of D. melanogaster testes, HeLa cells and the common house fly (Musca domestica).
It binds Protein A and is useful for immunoprecipitation assays and tissue staining in whole mount embryo and ovaries.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: 1 to 2 times per week
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG1
Name of Depositor
D Bopp, P Schedl
Deposited As
mouse (B cell); mouse (myeloma)
References

Bopp D, et al. Developmental distribution of female-specific Sex-lethal proteins in Drosophila melanogaster. Genes Dev. 5: 403-415, 1991. PubMed: 1900493

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