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MOLT-4

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價格:

編號:B165184

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

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甘油

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產品名稱

MOLT-4

商品貨號

B165184

Organism

Homo sapiens, human

Cell Type

T lymphoblast

培養基

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

acute lymphoblastic leukemia

Age

19 years

Gender

male

Applications

This cell line is a suitable transfection host.

Storage Conditions

liquid nitrogen vapor phase

Karyotype

This is a human cell line with the hypertetraploid chromosome number. The modal chromosome number was 95 occurring in 24% of cells. The rate of cells with higher ploidies was 0.8%. The 6q- and t(7;7) were paired and also common to all cells. The i(17q) was single and occurred only in some cells. Most normal chromosomes had four copies per cell except for the paired N7, X and Y, and two to three copies for N17.

Images

Derivation

MOLT-4 was derived from the same patient as the MOLT-3 cell line (ATCC CRL-1552). The line was established from cells taken from a patient in relapse.

Clinical Data

male

19 years

Antigen Expression

CD1 (49%), CD2 (35%), CD3 A (26%) B (33%) C (34%), CD4 (55%), CD5 (72%), CD6 (22%), CD7 (77%)

Genes Expressed

high levels of terminal deoxynucleotidyl transferase (TdT) are produced.

Cellular Products

high levels of terminal deoxynucleotidyl transferase (TdT) are produced

Tumorigenic

Yes

Effects

Yes, in untreated nude mice, anti lymphocyte serum treated mice and X-irradiated mice

Ref

Ohsugi Y, et al. Tumorigenicity of human malignant lymphoblasts: comparative study with unmanipulated nude mice, antilymphocyte serum-treated nude mice, and X- irradiated nude mice. J. Natl. Cancer Inst. 65: 715-718, 1980. PubMed: 6932523

Comments

The patient had received prior multidrug chemotherapy.

There is a G -> A mutation at codon 248 of the p53 gene.

P53 is not expressed.

The cells do not produce immunoglobulin or Epstein-Barr virus.

The line was originally contaminated with mycoplasma but has been cured with antibiotics.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.

Subculturing

Cultures can be maintained by addition or replacement of fresh medium. Start new cultures at 4 X 10⁵ cells/mL and subculture before the cell density reaches 2 X 10⁶ cells/mL.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C

STR Profile

Amelogenin: XY

CSF1PO: 11, 12, 13

D13S317: 12, 13

D16S539: 11, 14

D5S818: 12

D7S820: 8, 10, 11

THO1: 6, 8

TPOX: 8

vWA: 17, 18

Name of Depositor

J Minowada

Deposited As

Homo sapiens

References

Minowada J, et al. Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes. J. Natl. Cancer Inst. 49: 891-895, 1972. PubMed: 4567231

Mertelsmann R, et al. T-cell growth factor (interleukin 2) and terminal transferase activity in human leukemias and lymphoblastic cell lines. Blut 43: 99-103, 1981. PubMed: 6942897

Rodrigues NR, et al. p53 mutations in colorectal cancer. Proc. Natl. Acad. Sci. USA 87: 7555-7559, 1990. PubMed: 1699228

Sandstrom PA, Buttke TM. Autocrine production of extracellular catalase prevents apoptosis of the human CEM T-cell line in serum-free medium. Proc. Natl. Acad. Sci. USA 90: 4708-4712, 1993. PubMed: 8506323

Cross References

Nucleotide (GenBank) : AF146433 Homo sapiens cell-line MOLT-4 sodium/hydrogen exchanger isoform 1 mRNA, complete cds.

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