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MOLT-3
MOLT-3
規格:
價格:
編號:B165183
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
MOLT-3
商品貨號
B165183
Organism
Homo sapiens, human
Tissue
peripheral blood
培養基
Cell Type
T lymphoblast
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
acute lymphoblastic leukemia
Age
19 years
Gender
male
Storage Conditions
liquid nitrogen vapor phase
Karyotype
This is a hypertetraploid human cell line. The modal chromosome number was 97, occurring in 48% of cells. The rate of cells with higher ploidies was 2%. Three markers were common to all cells. They are: ?del(6) (q21), t(7;7), and an unidentifiable M5. The der(6) generally had three copies per cell, and the i(7q), two copies. The majority of normal chromosomes had 4 copies per cell. N8, N17, N19 and N20 had more than 4 copies in many cells. The X and Y chromosomes were two copies each per cell. Neither HSR chromosomes nor DM's were detected.
Derivation
MOLT-3 was derived from the same patient as the MOLT-4 cell line (ATCC CRL-1582).
This line was established from cells taken from a patient in relapse.
Clinical Data
male
19 years
Antigen Expression
CD1 (65%), CD4 (66%), CD5 (97%), CD7 (97%)
Genes Expressed
terminal deoxynucleotidyl transferase (TdT) activity is high
Cellular Products
terminal deoxynucleotidyl transferase (TdT) activity is high
Comments
No immunoglobulin or Epstein-Barr virus is detectable.
The patient had received prior multidrug chemotherapy.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Cultures are maintained by addition or replacement of fresh medium. Establish new cultures at 5 X 105 viable cells/mL and subculture at between 1 and 2 X 106 cells/mL.
Medium Renewal: Every 3 to 4 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Isoenzymes
AK-1, 0
ES-D, 1
G6PD, B
GLO-I, 1
Me-2, 0
PGM1, 1
PGM3, 0
Name of Depositor
J Minowada
Deposited As
Homo sapiens
References

Minowada J, et al. Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes. J. Natl. Cancer Inst. 49: 891-895, 1972. PubMed: 4567231

Mertelsmann R, et al. T-cell growth factor (interleukin 2) and terminal transferase activity in human leukemias and lymphoblastic cell lines. Blut 43: 99-103, 1981. PubMed: 6942897

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